Cyclobuta[1,2-b:3,4-b’]ditetracene – an analogue of nonacene with cyclobutadiene unit embedded in the central part has been synthesized by the combination of solution and on-surface chemistry. The atomic structure and electronic properties of the product on Au(111) have been determined by high resolution scanning tunnelling microscopy/spectroscopy corroborated by density functional theory calculations. Structural and magnetic parameters derived from theoretical calculations reveal that π conjugation is dominated by radialene-type contribution with an admixture of cyclobutadiene-like antiaromaticity.