The High Throughput Experimentation (HTE) laboratory is a facility that makes accessible to all ICIQ researchers the instrumentation and know-how to run a large number of reactions and analyze them in a short period of time.

The facility is set up to run different kinds of reactions in 24 or 96 well plates, including those that are air sensitive, reactions with reactive gases or reactions under pressure conditions. Moreover, the Laboratory is stocked with a large number of reagents, solvents, metals and ligands to run the reactions.

Regarding the analysis of the reactions, the lab is equipped with an NMR spectrometer and three chromatographs, a GC-MS, a UHPLC-MS and a supercritical CO2 chromatograph with diode array or MS detector.

To speed up the run processes, the lab is also equipped with specific software to prepare the experiments and analyze the results coming from the chromatographs.

This laboratory has received funding from Agencia Estatal de Investigación/Ministerio de Ciencia e Innovación (Severo Ochoa Excellence grant CEX2019-000925-S, MCIN/AEI/10.13039/501100011033).

Equipment

• 300 MHz NMR Spectrometer
  
  High speed NMR spectrometer to measure 13C and 1H, equipped with a 60 position autosampler.
• GC-MS
  
  Gas chromatograph coupled with a MS detector from Agilent. The device has a high capacity autosampler to work with several 96 analysis plates at the same time.
• Glove Box train
  
  Last generation glove box from MBraun with sixteen gloves. It’s a space to work under inert conditions that allows the storage of reagents and to set up, run and treat reactions. The box has several connections to provide services in the box: nitrogen flow, vacuum, chiller and electrical connections.
• Parallel Centrifugal Evaporator
  
  EZ-II Elite from Genevac. Device to evaporate the content of two 96 well plates at the same time, with the capability to evaporate solvents with boiling points up to 189ºC.
• Pressure reactors
  
  Reactors to run reactions under pressure conditions using 96 well plates.
• SFC chromatograph
  
  Supercritical CO2 chromatograph coupled with a DAD detector and the possibility to be coupled also with a MS detector and equipped with an autosampler to handle two 96 analysis plates. UPC2 from Waters.
• Tumble stirrers
  
  Highly efficient stirrers that ensure the perfect stirring of all the positions in 96 well plates.
• UHPLC chromatograph
  
  UHPLC coupled with DAD and MS detectors and an autosampler that handles two 96 analysis plates. Acquity from Waters.
• Well plates
  
  A wide collection of 96 and 24 well plates to run reactions under different conditions.