Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes

An emissive terbium complex has been conjugated to a C12 chain, Lys-Arg7, Arg7, a tetraguanidinium cation and human serum albumin; two-photon excitation at 720 nm facilitated microscopy studies revealing cell localisation profiles with the oligo-guanidinium conjugate localising in mitochondria but causing apoptotic cell death (IC5012 µM), the C12-amide complex giving rise to necrotic cell death in skin fibroblasts (IC50 8 µM) and the peptide conjugates and the methyl ester generating punctuate cytosolic intracellular distributions.

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